小尾寒羊黄体期和卵泡期生殖轴凋亡基因的表达研究及其与发情的关系

doi:10.11843/j.issn.0366-6964.2017.11.010

Abstract

Abstract:

In order to identify the differential expression levels of Bad,Bax,Bcl-2 and Bcl-xL in hypothalamus-pituitary-gonadal (HPG) axis tissues during luteal and follicular periods, and to study the association of these genes with year-round estrus in Small Tail Han sheep. Year-round estrous Small Tail Han ewes were selected as tested subjects,and real-time PCR method was used to detect Bad,Bax,Bcl-2 and Bcl-xL mRNA expression by using 18S RNA as reference gene in hypothalamus-pituitary-gonadal axis tissues during luteal and follicular periods. The serum estradiol and progesterone contents were measured by RIA at the corresponding time points. The results showed that Bad,Bax,Bcl-2 and Bcl-xL genes were all expressed in hypothalamus, pituitary and ovary tissues. The expression levels of Bad and Bcl-2 genes in pituitary and ovary tissues in the luteal phase were higher than those in the follicular phase (P<0.05). The expression levels of Bad and Bcl-2 genes in hypothalamus had no significant differences between the luteal phase and the follicular phase (P>0.05). The expression levels of Bax and Bcl-xL genes in hypothalamus, pituitary and ovary tissues had no significant differences between the luteal phase and the follicular phase (P>0.05). The concentration of progesterone in the luteal phase was significantly higher than that in the follicular phase (P<0.05), while the estradiol concentration had no significant difference between the 2 stages (P>0.05). The results indicated that the expression levels of Bad and Bcl-2 genes had great differences during the luteal and follicular periods of Small Tail Han sheep,which might regulate the stage transition from luteal to follicular periods, and the 2 genes were associated with estrus onset in Small Tail Han sheep.

CLC Number:

S826.2

CAO Xiao-han, LIU Qiu-yue, WANG Xiang-yu, DI Ran, HU Wen-ping, ZHANG Xiao-sheng, ZHANG Jin-long, ZENG Xian-yin, CHU Ming-xing. Expression Levels of Apoptotic Genes in HPG Axis during Luteal and Follicular Periods and Their Association with Estrus in Small Tail Han Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(11): 2091-2097.

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Expression Levels of Apoptotic Genes in HPG Axis during Luteal and Follicular Periods and Their Association with Estrus in Small Tail Han Sheep

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